Water Water Everywhere, and not a drop to drink!

Blog Action Day 2010: Water

Right now, almost a billion people on the planet don’t have access to clean, safe drinking water. That’s one in eight of us.

Unsafe water and lack of basic sanitation cause 80% of diseases and kill more people every year than all forms of violence, including war. Children are especially vulnerable, as their bodies aren’t strong enough to fight diarrhea, dysentery and other illnesses. The UN predicts that one tenth of the global disease burden can be prevented simply by improving water supply and sanitation.

But, water moves beyond just a human rights issue. It’s an environmental issue, an animal welfare issue, a sustainability issue. Water is a global issue, deserving a global conversation.

 

 

  • A Human Right: In July, to address the water crisis, the United Nations declared access to clean water and sanitation a human right over. But we are far from implementing solutions to secure basic access to safe drinking water. More Info »
  • 40 Billion Hours: African women walk over 40 billion hours each year carrying cisterns weighing up to 18 kilograms to gather water, which is usually still not safe to drink. More Info »
  • 38,000 Children a Week: Every week, nearly 38,000 children under the age of 5 die from unsafe drinking water and unhygienic living conditions. More Info »
  • Wars Over Water: Many scholars attribute the conflict in Darfur at least in part to lack of access to water. A report commissioned by the UN found that in the 21st century, water scarcity will become one of the leading causes of conflict in Africa. More Info »
  • Cell Phones vs. Toilets: Today, 2.5 billion people lack access to toilets, but many more have access to a cell phone. More Info »
  • Food Footprint: It takes 24 liters of water to produce one hamburger. That means it would take over 19.9 billion liters of water to make just one hamburger for every person in Europe. More Info »
  • Technology Footprint: The shiny new iPhone in your pocket requires half a liter of water to charge. That may not seem like much, but with over 80 million active iPhones in the world, that’s 40 million liters to charge those alone. More Info »
  • Fashion Footprint: That cotton t-shirt you’re wearing right now took 1,514 liters of water to produce, and your jeans required an extra 6,813 liters. More Info »
  • Bottled Water Footprint: The US, Mexico and China lead the world in bottled water consumption, with people in the US drinking an average of 200 bottles of water per person each year. Over 17 million barrels of oil are needed to manufacture those water bottles, 86 percent of which will never be recycled. More Info »
  • Waste Overflow: Every day, 2 million tons of human waste are disposed of in water sources. This not only negatively impacts the environment but also harms the health of surrounding communities.More Info »
  • Polluted Oceans: Death and disease caused by polluted coastal waters costs the global economy $12.8 billion a year. More Info »
  • Uninhabitable Rivers: Today, 40% of America’s rivers and 46% of America’s lakes are too polluted for fishing, swimming, or aquatic life.More Info »
  • Building Wells: Organizations like Water.org and charity: water are leading the charge in bringing fresh water to communities in the developing world. More Info »
  • Technology for Good: Do you want to measure how much water it took to make your favorite foods? There’s an app for that. More Info »
  • Conservation Starts at Home: The average person uses 465 liters of water per day. Find out how much you use and challenge your readers to do that same. More Info »
  • Keeping Rivers Clean: We can all take small steps to help keep pollution out of our rivers and streams, like correctly disposing of household wastes. More Info »
  • Drop the Bottle: Communities around the world are taking steps to reduce water bottle waste by eliminating bottled water. More Info »
  • Water, Sanitation and Hygiene in Schools: Students in developing countries lose 443 million school days each year due to diseases associated with the lack of water, sanitation and hygiene. Repeated episodes of diarrhea and worm infestations diminish a child’s ability to learn and impair cognitive development. More Info »
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    Published in: on October 15, 2010 at 5:40 am  Comments (1)  

    Take a Stand for an International Water Treaty to Provide Clean Water Everywhere

    SIGN the PETITION

     

    Water-Wrights United for Sovereign Human Rights

    Water-Wrights United for Sovereign Human Rights

     

    ~~~~~~~~~~~~
    Targeting:
    ~~~~~~~~~~~

    Ban Ki-Moon (UN Secretary-General)

    ~~~~~~~~~~~
    Sponsored by:
    ~~~~~~~~~~~

    United States Fund for UNICEF

    ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
    Across the globe, about 4,500 children die each day from unsafe water and lack of basic sanitation facilities. Over 90% of deaths from diarrhea diseases from unsafe water and sanitation occur in children under 5 years old.

    The poor are especially hard hit. An infant born in sub-Saharan Africa is 520 times more likely to die from disease than a child born in Europe or the United States.

    All told, more than 884 million of the world’s people still rely on drinking water sources that are unsafe.

    SIGN the PETITION

    Take action today in support of the UN’s work to supply clean, safe drinking water to the world’s poorest populations and urge UN Secretary-General Ban Ki-moon to continue the UN’s life-saving work bringing water and sanitation to developing nations.

    Through continued efforts of the United Nations and organizations like UNICEF, the world is currently on track to meet the Millennium Development Goal on water. With the exception of sub-Saharan Africa, all regions should meet their water targets — but only through continued dedication and efforts by the UN and member countries.

    Together with partners like UNICEF, we are on a path to provide clean, safe drinking water to millions around the world who need it most.

    UNICEF is on the front lines, doing whatever it takes to stop children dying from lack of access to clean, safe water and sanitation. UNICEF sponsors the UNICEF Tap Project which raises money each year in partnership with thousands of restaurants in the U.S. Every dollar from the TAP project supports UNICEF Water, Sanitation and Hygiene programs—saving and improving the lives of countless children in more than 100 countries around the globe.

    SIGN the PETITION

    ~~~~~~~~~~~~~ (more…)

    Vegan B12 BioAvailable in Shiitake Mushrooms

    …more on B12 content in Tempeh, Seaweeds, Organic Produce, and Other Plant Foods, below…

    Note* This article is an edit of a very important and comprehensive article found at http://www.veganhealth.org/b12/
    http://www.veganhealth.org/b12/plant#mush.
    I hope you find the information both educational and useful.
    Cheers! H. Alfred Goolsbee
    shiitake-on-logs

    shiitake-on-logs

    Summary: No food in Europe or the U.S. other than Japanese Nori, has been tested* for lowering MMA levels (*at the time of publication of this original article).

    another Important related link:
    https://backuppolymathium.wordpress.com/2010/10/10/vitamin-b12-for-vegans-confirmed/

    Thus, the discussion about whether Western vegans can get B12 from plant foods can, and probably should, end here (until proper research is conducted). Because so many plant foods have failed other tests that do not measure up to the MMA lowering test, and because there are so many false rumors being passed around, the studies of B12 in plant foods are examined in detail below.

    The only plant foods which have been tested for B12 activity using the gold standard of lowering MMA levels in humans are dried and raw nori from Japan. Dried nori made MMA status worse, indicating that it can reduce B12 status and can possibly harm people who are B12-deficient. Raw nori kept MMA levels about the same, indicating that it didn’t harm B12 status, but it did not help either.

    Of all the foods studied below, only tempeh in Indonesia or Thailand, dulse, Chlorella, raw nori, Aphanizomenon flos-aquae, and coccolithophorid algae warrant much further attention for providing B12. Unless these foods are shown consistently to correct B12 deficiency, vegans should not rely on them as a B12 source.

    Contents

    • Introduction
    • Plant Foods with Practically No Detectable B12 Analogue
    • Tempeh
    • Blue-Green Algae
      • Aphanizomenon Flos-aquae
      • Spirulina
    • Seaweeds (Macroalgae)
      • Various Seaweeds: Dulse Warrants Further Study
      • Chlorella
      • Nori
      • Coccolithophorid Algae
      • A Case of False Reporting on the Benefit of Seaweed and Fermented Foods
      • Genmai-Saishoku Paradox?
    • Soil and Organic Produce as a B12 Source for Vegans
      • B12 Analogue in Soil
      • Iranian Villagers
      • Organic Produce as a B12 Source for Vegans
        • Soybean Plants Absorb B12
        • Plants Absorb B12 Analogue When Fertilized with Cow Dung
        • Mushrooms and B12
        • Conclusion About Organic Produce as a B12 Source for Vegans
    • References

    Introduction

    It would be great to find a reliable plant source of B12 for vegans. One might get the opposite impression given my level of critique of some of these studies. My skepticism is only due to the potential harm from relying on a food that contains inactive B12 analogues which can make a B12 deficiency even worse.

    There has been a long history of misconceptions about which, if any, plant foods are sources of B12. Much of this stems from the methods of measuring B12 analogues. Other confusion stems from bacterial contamination that occurs in some foods but not others. Please see Measuring B12 in Plant Foods: Why the Confusion? for an explanation of the methods for for measuring B12 analogues in plant foods.

    Unlike animals, most, if not all, plants have no B12 requirement for any function, and therefore have no active mechanisms to produce or store B12. Many seaweeds have been shown to have B12 analogues.

    Most seaweeds are macroalgae, which are technically not plants. Some microalgae contain an enzyme that can use cobalamin, but also have an enzyme with the same function that does not require cobalamin in case it is not present. These macroalgae do not make their own cobalamin, but rather have a symbiotic relationship with cobalamin-producing bacteria (1). Note that I am purposefully using the term “cobalamin” rather than “vitamin B12” because it is not clear if these cobalamins are active vitamin B12 in humans.

    During the 1970s, two enzymes in plants (potatoes and bean seedlings) were found to respond to the addition of adenosylcobalamin (2, 3) (a co-enzyme form of B12). One explanation is that adenosylcobalamin provides some factor that is usable by these enzymes, but that adenosylcobalamin is not required by these plants for growth. Thus far, these plants have not been shown to counteract B12 deficiency symptoms (though I am not aware of any well-designed attempts as it is assumed that they do not contain B12). It is probably safe to assume that many vegans who have developed severe B12 deficiency ate potatoes and beans.

    There are some rumors, though no evidence of which I am aware, that if you let organic produce, such as carrots, sit at room temperature for a few hours, bacteria on the surface of the carrots will produce B12. For this to happen, specific species of bacteria would be required, as would cobalt. Until there is research showing that such a method can lower MMA levels, such produce should not be considered to provide B12.

    Table 1. Foods with No Detectable B12 Analogue

    Amesake rice4
    Barley miso4
    Miso5
    Natto5
    Rice miso4
    Shoyu4
    Tamari4
    Umeboshi prunes4
    Various fruits, vegetables, nuts,seeds, & grains5

    Plant Foods with Practically No Detectable B12 Analogue

    Various studies have tested the foods in Table 1 for B12 analogues and found none. To my knowledge, other than in Mozafar’s studies (below) in which B12 and cow dung were carefully added to the soil of potted plants, no published study has shown any B12 analogues in any of these foods.

    Table 2. B12 Analogue Content (µg/30 g) of Various Foods

    Netherlands4 Thailand5, 6
    Assay IF IF or R-proteinA
    Fermented soybean 0.15
    Barley malt syrupSourdough bread

    Parsley

    Shiitake mushrooms

    .006-0.1Only info given
    Dried fermented soybean 0.01
    Tofu ND 0.02
    Soybean paste 0.03
    Soy sauce .01B
    A – Used an assay method by Lau et al.32 (1965) which uses R-protein or IFB – µg/30 ml

    IF – Intrinsic factor Assay

    ND – None Detected

    Table 2 shows the B12 analogue content of various plant foods:

    As you can see, there are very small amounts, if any. Since the amounts are so small, any inactive analogues should not significantly interfere with an individual’s active B12 from other sources, and if the analogue is active B12, it will not provide much. Thus, these foods should neither add to, nor detract from, a vegan’s B12 status.

    Tempeh

    For a long time, tempeh has been said to contain B12. Table 3 shows the results of measuring B12 analogue in various tempehs.

    Table 3. B12 Analogue Content (µg/30 g) of Tempehs
    Netherlands4 USA7 Indonesia5,6
    Assay IF IF IF or R-proteinA
    tempeh ND .02C .054-1.2B
    A – Used an assay method by Lau et al.32 (1965) which uses R-protein or IFB – 10 commercial tempeh samples purchased from various markets in Jakarta, Indonesia

    C – Cooked for 60 minutes

    IF – Intrinsic factor

    ND – None Detected

    The studies in the USA and in The Netherlands showed little to no B12 analogue.

    In contrast, Areekul et al. (6) (1990, Indonesia/Thailand) found more significant amounts of B12 analogue. Tempeh production requires molds belonging to the genus Rhizopus. These were found not to produce B12 analogues in Areekul et al.’s study. Rather, a bacterium, identified as Klebsiella pneumoniae, was isolated from the commercial tempeh starter and determined to be the B12 analogue source. This confirmed Albert et al.’s (8) (1980) finding that the Klebsiella genera could produce B12 analogues. In Albert’s study, the analogue was thought to be active B12. Whether the analogues found by Areekul et al. were the same as in Albert’s study is not known. Given that K. pneumoniae is not required for tempeh production, we can conclude that the B12 analogue found in the tempehs in Indonesia were due to bacterial contamination (though apparently common there). Tempeh in Europe and the U.S. cannot be relied on as a source of B12. Until tempeh in Indonesia is shown to reduce MMA levels, it should not be relied upon there, either.

    Blue-Green Algae

    Blue-green algae are also known as cyanobacteria, blue-green bacteria, and cyanophyta. They are not actually algae, but rather organisms with characteristics of both bacteria and algae. They can peform photosynthesis and are thought to be the ancestors to chloroplasts in algae and plants.

    Aphanizomenon Flos-aquae

    Cell Tech and some other companies market algae from Klamanth Lake in Oregon. Cell Tech markets a species, Aphanizomenon flos-aquae, they call Super Blue Green Algae (SBGA). On April 16, 2003, Cell Tech’s now defunct website stated:

    “Is the vitamin B12 in SBGA bioavailable and bioactive? Yes. The Super Blue Green Algae (SBGA) strain, Aphanizomenon flos-aquae, has been tested by Lancaster Labs for B12 analog levels using microbiological testing methods that are comparable to methods 952.20 and 960.46 of the Association of Analytical Chemists (AOAC). Unlike other plant foods such as Spirulina, which contain corrinoids with virtually no vitamin B12 activity, Aphanizomenon flos-aquae is a reliable source for vegetarians seeking to supplement their diets with a bioactive form of this important nutrient.”

    However, test methods 952.20 and 960.46 use Lactobacillus leichmannii (9), which can measure non-B12 corrinoids (10). See the table Test Organisms for B12 Microbiological Assays in Measuring B12: Why the Confusion? Thus, it can only be concluded that Cell Tech’s SBGA contains B12 analogues whose activity is yet to be determined.

    2010 Update: It appears that Cell Tech is now the company, Simplexity Health, and is no longer touting SBGA as a source of vitamin B12.

    In a 2009 study from Italy (11), researchers gave Aphanizomenon flos-aquae to 15 vegans. First there was a washout period in which the vegans took no supplemental B12 for 3 months. They were then given 6 capsules of Klamanth Algae from Nutratec (which also contained digestive enzymes to help absorption).

    Table 4. Supplementation with Aphanizomenon flos-aquae
    Marker Baseline 3 mosA 6 mosB
    Homocysteine (µmol/l) 13.7 15.2^ 12.0*
    Serum B12 (pg/ml) 259 196^ 237
    Folate (ng/ml) 11.0 10.9 12.5
    ^Statistically significant difference from baseline.*Statistically significant difference from 3 months.

    The results, seen in Table 4, show that the average homocysteine level went down. The authors believe this is an indication that Aphanizomenon flos-aquae is a source of active vitamin B12, and that it “warrants further larger, and longer-term randomized trials to confirm such preliminary conclusions.”

    Here are some problems with the study:

    1. The authors state in the paper that homocysteine is the most reliable marker for B12 activity, but it is not. Homocysteine levels can be affected by folate intake and, to a lesser extent, vitamin B6. Methylmalonic acid levels are the most reliable marker for B12 activity. This is well known and uncontroversial, so it is odd that the researchers did not know this.
    2. The authors noted that vitamin B6 could not have reduced the homocysteine levels because the algae has very little. They also said that folate levels could not have affected them, but in looking at the results, folate levels did increase (even though the difference was not statistically signifcant).
    3. The homocysteine levels of these vegans started out pretty high, and when the study ended they were still much too high. A safer level is closer to 6 – 8 µmol/l.
    4. One subject’s homocysteine level increased, and one subject’s homocysteine level that was about 10 µmol/l did not respond to the aglae supplementation.
    5. The researchers obtained the algae directly from a company that produces it. It would have been more reassuring if the algae were purchased in a store where the company didn’t realize it was going to be tested.

    In another study from Italy (2002) (12), vegetarians had really high homocysteine levels (25 µmol/l). This is much higher than almost all other studies, which makes one wonder what’s going on in Italy.

    In conclusion, it appears that Aphanizomenon flos-aquae might provide some vitamin B12 activity in humans. On the other hand, it did not succeed in lowering homocysteine to an ideal level whereas vitamin B12 supplements do succeed at doing so. At this time, it would be prudent not to rely on it for optimal health.

    Spirulina

    Table 5 shows the B12 analogue content (µg/30 g) of various spirulina batches:

    Table 5. B12 Analogue Content (µg/30 g) of Spirulina

    Netherlands4 USA13 Japan14
    Assay IF L. leich. IF L. leich. L. leich. IF PC
    Spirulina 14.5 67 36.7 193.1 73 2.5 0.44
    Spirulina 6 35.3 38 1.9 0.32
    Spirulina 1.67 8.7 44 5.2 0.88
    IF – Intrinsic factor AssayPC – Paper Chromotography Assay

    The wide range of B12 analogues from one measurement method to another indicates that spirulina has a wide variety of different analogues, many of which are inactive. Some may interfere with B12 activity in humans.

    Seaweeds (Macroalgae)

    Various Seaweeds: Dulse Warrants Further Study

    Table 6. B12 Analogue Content (µg/30 g) of Various Seaweeds

    Netherlands4 USA7
    Assay IF L. leich. IF
    Arame 0.042
    Dulse (Palmaria palmata) 3.9 3
    Hijiki < .006 < .006
    Kelp 1.2 0.12
    Kombu 0.84 0.018 .57-1.3A
    Wakame 1.4 0.009 1.29B
    IF – Intrinsic factor AssayA – Range of 5 samples of 3 different brands, with 3 samples cooked for 60 minutes

    B – Cooked for 60 minutes

    Table 6 shows the B12 analogue content of arame, dulse, hijiki, kelp, kombu, and wakame per 30 g of seaweed. Please note that 30 g is a lot of seaweed. A serving size would be closer to 3 grams. Seaweeds also tend to be very high in iodine, which can cause problems at high intakes. So, consuming mass quantities of seaweed is unadvisable.

    The only seaweed in this list that warrants further study is dulse (also spelled “dulce”), which contains .3 to .39 µg of B12 analogue per 3 g serving. Unless dulse is eventually shown to lower MMA levels, it should not be considered a source of active B12.

    Chlorella

    Pratt & Johnson (15) (1968, USA) studied numerous batches of chlorella and occasionally found amounts of B12 analogue that were in the range of error for the test method. In other words, they were not able to detect practical amounts. They noted that their extraction processes might not have been adequate though they used many different methods. They also noted that their synthetic medium on which the chlorella was grown might have interfered with B12 analogue synthesis.

    Kittaka-Katsura et al. (16) (2002, Japan) measured B12 analogue levels in Chlorella using both a Lactobacillus leichmannii ATCC 7830 and an intrinsic factor assay. Both methods showed about the same amount of B12 analogue, listed in Table 7 below:

    Chen and Jiang (17) (2008, Taiwan) used capillary electrophoresis to detect cyanocobalamin and hydroxocobalamin in chlorella. Capillary electrophoresis is a relatively new method that should be able to detect the exact structure of a cobalamin analogue. They found considerable amounts of cyanocobalamin in two samples of Chlorella, with negligible amounts of B12 analogues.

    Table 7. B12 Analogue Content (µg/30 g) of Chlorella

    USA15 Japan16 Taiwan17
    Assay E. gracilis & O. malhamensis L. leich. IF Capillary Electrophoresis
    Chlorella vulgaris ND
    Chlorella pyrenoidosa ND
    Chlorella sp. 60.4 – 85.7 60.1 – 63.5 3.9 11.4
    IF – Intrinsic Factor

    Until chlorella is tested on humans to determine whether it lowers MMA levels, it should not be considered a reliable source of vitamin B12, especially since the study by Pratt & Johnson (15) showed no vitamin B12.

    Nori

    Table 8 below shows the B12 analogue content of various nori types and batches:

    Table 8. B12 Analogue Content (µg/30 g) of Nori

    Netherlands4 Japan18 Japan19
    Assay IF L. leich. L. leich. IF E. Coli 215 IF PC
    Nori (P. umbilica) 3.6
    Nori (P. tenera) 5.4-12.9A
    Nori (purple, Porphyra sp) 9.7 7.5
    Nori (green, Enteromorpha sp) 19.1 21
    Nori (P. tenera) 20.1 20.1
    Dried nori (P. tenera) 4.3 < 4.3 1.5
    Raw nori (P. tenera) 3.8 ~ 3.8 2.7
    A – Range of 3 different samplesIF – Intrinsic factor Assay

    PC – Paper Chromotography Assay

    Various batches of nori were found to contain significant amounts of B12 analogue. One study verified the molecular weight through paper chromotagraphy, indicating that there is a good chance that some of this B12 is active. Yamada et al. (20) (1996, Japan) determined that nori contains what they considered to be active B12 analogues using various assays and methods (results not reported here). However, in light of Dagnelie’s results, Yamada et al. (19)(1999, Japan), decided to test the nori to see if it could reduce methylmalonic acid (MMA) levels, the gold standard for determining the B12 activity of a food:

    Raw nori was purchased within 48 hours of harvesting. Dried nori was purchased from a store. Inactive vs. active B12 was determined by IF assay and confirmed by paper chromatography. 10 people (all nonvegetarian) were then studied. The results are shown in Table 9.

    Table 9. Yamada et al.’s19 Study of Nori’s Impact on Urine MMA Levels

    N B12 found tobe analogue Amount Duration uMMA
    Dried nori 6 65% 40 g (20 sheets)A 6-9 days increased 77% SS
    Raw nori 4 27% 320 g/day A 3-6 days increased 5% NS
    A – Equivalent amountsNS – Not statistically significant

    SS – Statistically significant

    N – Number of people tested

    The results indicate that B12 in raw nori can be changed into harmful inactive B12 analogues by drying, and that dried nori decreases B12 status. Yamada et al. said that although dried nori cannot be used as a B12 source, in small amounts it is not harmful. However, they believe that raw nori is an excellent source of genuine B12.

    I disagree with their conclusion that raw nori is an excellent source of active B12. While eating raw nori, the subjects’ uMMA levels increased 5%. While this was not enough of an increase to be statistically significant, it indicates that the raw nori did not improve B12 status (which would have required MMA levels to drop, rather than increase). This study showed that this batch of raw nori did not have enough inactive B12 versus active B12 analogue to be considerably detrimental, but it did not prove any benefit.

    The study by Yamada et al. was further confounded by adding valine (an amino acid that can be converted into MMA when B12 is deficient) to the subjects’ diet in order to increase MMA levels so that a difference could be seen. The valine did not appear to do this when given without the nori, and no control groups were included, making the results even more difficult to interpret.

    Other studies have measured the B12 analogue content of nori, but without testing to see if they could lower MMA levels:

    Coccolithophorid Algae

    Table 10. B12 Analogue Content (µg/30 g) of Coccolithophorid Algae

    Japan21
    Assay IF L. delbrueckii
    Coccolithophorid algae (Pleurochrysis carterae) 37.6 37.6A
    A – Study said the amount was “identical” to that found with IF; the number was not actually givenA – Equivalent amounts

    IF – Intrinsic Factor

    Coccolithophorid algae (Pleurochrysis carterae) is being used in Japan as a calcium supplement. Miyamoto et al. (21) (2001, Japan) analyzed it for B12 analogue content:

    They tried to verify whether it is an active form of B12 through liquid chromatography, and thought that is was. B12 analogue remained stable for 6 months of storage. They later followed up with a second study on coccolithophorid algae (22), but still did not test it to see if it can lower MMA levels in humans.

    This algae deserves further attention to see if it can lower MMA levels.

    A Case of False Reporting on the Benefit of Seaweed and Fermented Foods

    Specker et al. (7) (1988, USA) reported a macrobiotic mother of an infant with a uMMA of 146 µg/mg who modified her diet by increasing her consumption of seaweeds and fermented foods. The infant’s uMMA dropped to 27 µg/mg in 2 months and to 13 µg/mg in 4 months. It was later discovered that this mother had also eaten fish and clam broth which were probably responsible for the improvement rather than the seaweeds and fermented foods (23). Specker et al. stated, “The vegetarian community we worked with believed fermented foods in their diet contained adequate amounts of vitamin B12.” However, on analysis, the fermented foods were shown not to have B12. (7).

    Genmai-Saishoku Paradox?

    Suzuki (24) (1995, Japan) studied 6 vegan children eating a genmai-saishoku (GS) diet, which is based on high intakes of brown rice and contains plenty of sea vegetables, including 2-4 g of nori per day (“dried laver”); as well as hijiki, wakame, and kombu. The foods are organically grown and many are high in cobalt (buckwheat, adzuki beans, kidney beans, shiitake, hijiki). Serum B12 levels of the children are shown in Table 11:

    Table 11. Results of Suzuki24.

    age (yrs) years vegan serum B12
    7.1 4.4 520
    7.7 4.4 720
    8.6A 8.6 480
    8.8A 8.8 300
    12.7 10 320
    14.6 10 320
    average 443 (± 164)
    A – Exclusively breast-fed until 6 months old. Mothers had been vegan for 9.6 and 6.5 yrs prior to conception. Both mothers consumed 2 g of nori per day.

    None of the many measurements between the vegans and 4 nonvegan controls were significantly different, including serum B12, MCV, and iron indicators. MMA and homocysteine levels were not measured. Some suggestions as to how the vegans got their B12 are:

    • From nori or the other seaweeds. The nori was most likely dried.
    • Small amounts of B12 from B12 uptake or contamination of plants grown in manure.
    • B12 from their mothers’ stores.

    These results are both interesting and perplexing. The serum B12 levels are easy to explain as possibly being inactive B12 analogues. But it is particularly impressive that the eight-year-olds were doing well given that their mothers had been vegan for some time, supposedly without B12-fortified foods or supplements. Unfortunately, many vegan children have not had the same positive results, and until more is known about the GS children’s diets, this study should be considered an unsolved mystery.

    If these children were my own, I would make sure they started to get at least a modest B12 supplement to ensure their continued good health.

    Soil and Organic Produce as a B12 Source for Vegans

    It is common in vegan circles to hear that if your produce has soil on it and you do not wash the produce before eating it, bacteria that lives in the soil and on the produce will provide B12. It is also claimed that in today’s world, our food supply is very sanitized whereas in the past, vegan humans would have received plenty of B12 from the unsanitized produce. What is the evidence for these claims?

    B12 Analogue in Soil

    There is a one paragraph report often cited in vegan literature for showing that B12 is found in the soil. Robbins et al. (25) (1950, New York) used Euglena gracilis var. bacillari as a microbiological assay for vitamin B12 “or its physiological equivalent.” A considerable proportion of bacteria and actinomycetes (molds) in the soil were found to synthesize B12 analogues. B12 analogues were also found in the roots of plants (.0002-.01 µg B12/g of fresh material). Some stems had some B12 analogue, but leaves and fruit generally did not. B12 analogue was also found in pond water and pond mud. There was no indication in the report as to how many different soils were tested, but the impression was that it was all in one local area. There is no way to know whether these molecules were active or inactive B12 analogues.

    Iranian Villagers

    Herbert (26) reported a group of “vegan” Iranians growing plants in night soil (human manure). The vegetables were eaten without being carefully washed and the amount of B12 was enough to prevent deficiency. However, for this information, Herbert cites Halstead et al. (1959) (27), who do not mention these Iranians in their paper. Herbert possibly meant to cite a 1960 paper by Halstead et al. (28) which reported that some Iranian villagers with very little animal product intake (dairy once a week, meat once a month) had normal B12 levels. None had megaloblastic anemia. Their average B12 level was 411 pg/ml which was quite high considering their diet. The authors speculated this could be because their diets, which were very low in protein, allowed for B12-producing bacteria to ascend into the ileum where the B12 could be absorbed. They also speculated that because they lived among their farm animals and their living areas were littered with feces, they picked up enough B12 through contamination.

    Organic Produce as a B12 Source for Vegans

    Soybean Plants Absorb B12

    Mozafar & Oertli (29) (1992, Switzerland) added cyanocobalamin to the soil of soybean plants in amounts ranging from 10 to 3200 µmol/l. Using an intrinsic factor assay, 12-34% of the B12 was absorbed by the plants. 66-87% of the absorbed vitamin remained in the roots and the rest was transported to the various other parts, mainly the leaves. Mozafar points out that the concentrations of B12 in the soil used in this study were many times higher than the reported vitamin concentration in soil solution (.003 µmol/l) measured by Robbins (25).

    Plants Absorb B12 Analogue When Fertilized with Cow Dung

    Table 12. B12 Analogue in Soil30

    Sample 1(µg/kg) Sample 2(µg/kg)
    Synthetically fertilized soil 9 5
    Organically fertilized soilA 14 10
    A – Treated with organic fertilizer once every 5 years

    In light of the above results, Mozafar (30) (1994, Switzerland) then studied how the B12 levels in plants are affected by adding cow dung to the soil. An assay using pig intrinsic factor was used to measure the B12 analogue. The study looked at the B12 analogue content of both organically fertilized soil and plants.

    Two samples were taken from soil that had been treated with organic fertilizer every 5 years over the previous 16 years. The B12 analogue content in these samples was compared to soil that had only synthetic fertilizer applied. Results are shown in Table 12.

    Table 13. B12 Analogue (ng/g) in Plants30

    Nothing Added to Soil “Organic” (10 g Dry Cow Manure Added)
    Soybeans 1.6 2.9
    Barley kernels 2.6A 9.1A
    Spinach 6.9B 17.8B
    A,B – Statistically significant difference between groups with same letters

    Soybean, barley, and spinach plants were then grown in pots of 2.5 kg of soil. 10 g dry cow manure was added to each pot. Plant parts were thoroughly washed to remove any soil before B12 was measured. Table 13 shows the results.

    Further analysis showed that most or all of the B12 analogue in the plants was unbound. Mozafar concluded that plant uptake of B12 from the soil, especially from soil fertilized with manure, could provide some B12 for humans eating the plants, and may be why some vegans, who do not supplement with B12, do not develop B12 deficiency.

    Does this mean that organic foods are a good source of B12? No. These studies show that when B12 analogues are placed in the soil, plants can absorb them.

    Mushrooms and B12

    Table 14. B12 in Mushrooms

    Button Cup Flat
    Most
    Cap 1005 567 161
    Flesh 233 83 84
    Stalk 17 255 465
    Peel 217 1015 354
    Total (ng / 400 g) 1472 1920 1064
    ng / Cupa 257.60 336.00 186.20
    mcg / Cup 0.26 0.34 0.19
    Cups to meet RDA 9.32 7.14 12.89
    Least
    Cap 11 8 17
    Flesh 4 7 4
    Stalk 11 7 12
    Peel 36 20 68
    Total (ng / 400 g) 62 42 101
    ng / Cupa 10.85 7.35 17.68
    mcg / Cup 0.01 0.01 0.02
    Cups to meet RDA 221.20 326.53 135.79
    aAssume 70 g per Cup

    In 2009, a paper was published looking at the B12 analogue content of mushrooms in Australia (31). The authors used chromatography and mass spectrometry to determine whether the B12 was an active form, and they believed that it was.

    Table 14 shows the B12 analogue content of the batches of each mushroom containing the most B12 and the batches containing the least.

    Assuming that the B12 is active analogue, it would take anywhere from 7 to 326 cups of mushrooms to meet the RDA.

    As for the source of the B12, the authors were not sure, but they said:

    The high concentration of vitamin B12 in peel suggests that it was not synthesized within the mushrooms but was either absorbed directly from the compost or synthesized by bacteria on the mushroom surface. The latter is more likely because mushrooms have no root system to take up the vitamin in the compost as is the case with the uptake of vitamins by root plants from the soil containing fertilizers.

    Conclusion About Organic Produce as a B12 Source for Vegans

    Unless uncleaned, organic produce is shown to lower MMA levels, it is unjustified to claim that B12 can be obtained in such a manner, or to claim with certainty that humans have ever relied on it as a source of B12.

    Only until organic foods are chosen randomly from markets and grocery stores throughout the country (or world) and are consistently shown to decrease MMA levels will someone not be taking a considerable risk in relying on organic foods for B12. This article documents many vegans suffering from B12 deficiency, and it is safe to assume that many of them consumed significant amounts of organic foods.

    Additional note: The vegan movement is typically not aiming for a world where there are enough cows to produce a significant amount of manure for fertilizer.

    I hope you find the information both educational and useful. 
    Cheers! H. Alfred Goolsbee

    References

    1. Smith AG, Croft MT, Moulin M, Webb ME. Plants need their vitamins too. Curr Opin Plant Biol. 2007 Jun;10(3):266-75. Epub 2007 Apr 16. Review.

    2. Poston JM. Coenzyme B12-dependent enzymes in potatoes: leucine 2,3-aminomutase and methylmalonyl-coa mutase. Phytochemistry. 1978;17:401-402.

    3. Poston JM. Leucine 2,3-aminomutase: a cobalamin-dependent enzyme present in bean seedlings. Science. 1977;195:301-302.

    4. van den Berg H, Dagnelie PC, van Staveren WA. Vitamin B12 and Seaweed. Lancet Jan 30, 1988.

    5. Areekul S, Churdchu K, Pungpapong V. Serum folate, vitamin B12 and vitamin B12 binding protein in vegetarians. J Med Assoc Thai 1988 May;71(5):253-7.

    6.Areekul S, Pattanamatum S, Cheeramakara C, Churdchue K, Nitayapabskoon S, Chongsanguan M. The source and content of vitamin B12 in the tempehs. J Med Assoc Thai 1990 Mar;73(3):152-6.

    7. Specker BL, Miller D, Norman EJ, Greene H, Hayes KC. Increased urinary methylmalonic acid excretion in breast-fed infants of vegetarian mothers and identification of an acceptable dietary source of vitamin B-12. Am J Clin Nutr 1988 Jan;47(1):89-92.

    8. Albert MJ, Mathan VI, Baker SJ. Vitamin B12 synthesis by human small intestinal bacteria. Nature 1980;283(Feb 21):781-2.

    9. Helrich K, ed. Official Methods of Analysis, Volume 2: Food Composition; Additives; Natural Contaminants, 15th Edition. Arlington, VA: Association of Official Analytical Chemists, Inc; 1990.

    10. Schneider Z, Stroinski A. Comprehensive B12. New York: Walter de Gruyter, 1987.

    11. Baroni L, Scoglio S, Benedetti S, Bonetto C, Pagliarani S, Benedetti Y, Rocchi M, Canestrari F. Effect of a Klamath algae product (“AFA-B12”) on blood levels of vitamin B12 and homocysteine in vegan subjects: a pilot study. Int J Vitam Nutr Res. 2009 Mar;79(2):117-23.

    12. Bissoli L, Di Francesco V, Ballarin A, Mandragona R, Trespidi R, Brocco G, Caruso B, Bosello O, Zamboni M. Effect of vegetarian diet on homocysteine levels. Ann Nutr Metab. 2002;46(2):73-9.

    13. Herbert V, Drivas G. Spirulina and Vitamin B12. JAMA. 1982;248(23):3096-7.

    14. Watanabe F, Katsura H, Takenaka S, Fujita T, Abe K, Tamura Y, Nakatsuka T, Nakano Y. Pseudovitamin B(12) is the predominant cobamide of an algal health food, spirulina tablets. J Agric Food Chem. 1999 Nov;47(11):4736-41.

    15. Pratt R, Johnson E. Deficiency of vitamin B12 in Chlorella. J Pharm Sci. 1968 Jun;57(6):1040-1.

    16. Kittaka-Katsura H, Fujita T, Watanabe F, Nakano Y. Purification and characterization of a corrinoid compound from Chlorella tablets as an algal health food. J Agric Food Chem. 2002 Aug 14;50(17):4994-7. (Abstract)

    17. Chen JH, Jiang SJ. Determination of cobalamin in nutritive supplements and chlorella foods by capillary electrophoresis-inductively coupled plasma mass spectrometry. J Agric Food Chem. 2008 Feb 27;56(4):1210-5. Epub 2008 Feb 2.

    18. Watanabe F, Takenaka S, Katsura H, Masumder SA, Abe K, Tamura Y, Nakano Y. Dried green and purple lavers (Nori) contain substantial amounts of biologically active vitamin B(12) but less of dietary iodine relative to other edible seaweeds. J Agric Food Chem. 1999 Jun;47(6):2341-3.

    19. Yamada K, Yamada Y, Fukuda M, Yamada S. Bioavailability of dried asakusanori (porphyra tenera) as a source of Cobalamin (Vitamin B12). Int J Vitam Nutr Res. 1999 Nov;69(6):412-8.

    20. Yamada S, Shibata Y, Takayama M, Narita Y, Sugawara K, Fukuda M. Content and characteristics of vitamin B12 in some seaweeds. J Nutr Sci Vitaminol (Tokyo). 1996 Dec;42(6):497-505. (Abstract)

    21. Miyamoto E, Watanabe F, Ebara S, Takenaka S, Takenaka H, Yamaguchi Y, Tanaka N, Inui H, Nakano Y. Characterization of a vitamin B12 compound from unicellular coccolithophorid alga (Pleurochrysis carterae). J Agric Food Chem. 2001 Jul;49(7):3486-9.

    22. Miyamoto E, Watanabe F, Takenaka H, Nakano Y. Uptake and physiological function of vitamin B12 in a photosynthetic unicellular coccolithophorid alga, Pleurochrysis carterae. Biosci Biotechnol Biochem. 2002 Jan;66(1):195-8. (Abstract)

    23. Dagnelie PC, van Staveren WA, van den Berg H. Vitamin B-12 from algae appears not to be bioavailable. Am J Clin Nutr. 1991;53:695-7.

    24. Suzuki H. Serum vitamin B12 levels in young vegans who eat brown rice. J Nutr Sci Vitaminol 1995;41:587-594.

    25. Robbins WJ, Hervey A, Stebbins ME. Studies on Euglena and vitamin B12. Science 1950(Oct 20):455.

    26. Herbert V. Vitamin B-12: plant sources, requirements, and assay. Am J Clin Nutr. 1988;48:852-8.

    27. Halsted JA, Carroll J, Rubert S. Serum and tissue concentration of vitamin B12 in. certain pathologic states. N Engl J Med. 1959;260:575-80.

    28. Halsted JA, Carroll J, Dehghani A, Loghmani M, Prasad A. Serum vitamin B12 concentration in dietary deficiency. Am J Clin Nutr. 1960 May-Jun;8:374-6.

    29. Mozafar A, Oertli JJ. Uptake of microbially-produced vitamin (B12) by soybean roots. Plant and Soil. 1992;139:23-30.

    30. Mozafar A. Enrichment of some B-vitamins in plants with application of organic fertilizers. Plant & Soil. 1994;167:305-311.

    31. Koyyalamudi SR, Jeong SC, Cho KY, Pang G. Vitamin B12 is the active corrinoid produced in cultivated white button mushrooms (Agaricus bisporus). J Agric Food Chem. 2009 Jul 22;57(14):6327-33. PubMed PMID: 19552428.

    32. Lau KS, Gottleib C, Wasserman LR, Herbert V. Measurement of serum B12 level using radioisotopes dilution and coated charcoal. Blood 1965;26:202-8.

    Vitamin B12 for Vegans Confirmed

    Viable Vitamin B12 for Vegans available for centuries as common component of Japanese daily diet ~ Shiitake Mushroom

    Consider this: Do mushrooms require B12? Why would mushrooms adsorb and absorb the B12? Is this an accident of Nature;  or ~ is DNA much more intelligent than we realize?


    In AUSTRALIA ~ University of Western Sydney completed ground-breaking research on the B12 content of button mushrooms.

    In JAPAN ~ According to Dr. Kanichi Mori, Shiitake mushrooms have more vitamin B12 than milk and fish.

    Brief:Certain types of Mushrooms are unique in that they contain Vitamin B12, something that vegetables can’t produce at all.  Since B12 is mainly of animal origin, deficiency is commonly associated with vegetarian diets.  Mushrooms were found to contain 0.32-0.65 mg per gram of B12, allowing just 3 g of fresh mushrooms to provide the RDA of this vitamin.  Vegetarians may find this a useful way of getting this important nutrient. ~ more at http://www.polymathium.com ~ This is possibly due to a subtle, potential symbiotic relationship that occurs between the three parties involved… humans, mushrooms, and B12 producing bacterium.


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    Fasting on Fruit Juice ~ Day 31

    Hi there. Welcome to the Polymathium. My name is Henry Alfred Goolsbee; and I’ve been a vegan-vegetarian for over 40 years. This year for my (2009-2010) New Year’s resolution activity, I’m writing sequels to my previous books and fasting.  I’m performing a juice fast with the intent of writing a treatise on fasting and the ancient origins of the science of manufacturing ORMUS, using methods that most probably evolved along with the art soap making. I’ve been consuming approx. 90% fruit juice and 10% vegetable juice for over 4 weeks now. But I’ve only just begun! I’m aiming for 90~120 days at least.

    ~ Polymathius ~ A Compassionate Man of Ahimsa

    ~ Polymathius ~ A Compassionate Man of Ahimsa

     

    Today, (Day 31:) I enter into the 2nd month of fasting on liquids. I am fasting as an act of gratitude; to acknowledge the bounty and blessings that Gaia, our Mother Earth bestows upon us, moment to moment; and to remind others that we all share a common responsibility to give back to our Mother The Earth and our Natural Environment.

     

    Sustainability requires more than political activism. Sustainability begins at home and at the office, in our factories and our places of worship.

    Day 31: I feel alert and energized. I sleep well and wake up early, refreshed and looking forward to a new day. I’ve been consuming 90% fruit juice and 10% vegetable juice for over 4 weeks now.

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    Polymathium ~ What is it?

    The term Polymathium™ is a word coined* as a compliment to the term polymath.

    Wherein polymath is the critical component:
    Polymathium is a combination of the words Polymath, Colloquium and Auditorium.

    ~ Polymathius ~ A Compassionate Man of Ahimsa

    ~ Polymathius ~ A Compassionate Man of Ahimsa

     

    Polymathium~ a Colloquium for the Polymath in all of us.

    The Polymathium…is a place where classically minded people can congregate in cyberspace to discuss the “little things” (as opposed to those”big questions”) that make life worth living… returning to the roots of Philosophy, as it were.A polymath (Greek πολυμαθής, polymathēs, “having learned much”) is a person whose expertise spans a significant number of different subject areas. In less formal terms, a polymath (or polymathic person) may simply be someone who is very knowledgeable. Most ancient scientists were polymaths by today’s standards.

    ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
    Famous Polymathes of Ancient Times

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